Regulatory

Part:BBa_K944000:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-25)

Putative Promoter Induced by Cyanide Compounds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is known to be in the cyanase operon from Pseudomonas pseudoalcaligenes CECT5344 between the cynF which is the transcriptional regulator and CynA, but its specific sequence is unknown, so our team use the sequence between this two genes to be sure that we get our inducible promoter.


Source

This part came from the genomic sequence Pseudomonas pseudoalcaligenes CECT5344 and was synthesized by IDT technologies.

References

Luque-Almagro V. M., Huertas M. J., Sáez L. P., Luque-Romero M. M., Moreno-Vivián C., Castillo F., Roldán M. D., Blasco R. (2008). Characterization of the Pseudomonas pseudoalcaligenes CECT5344 cyanase, an enzyme that is not essential for cyanide assimilation. Appl. Environ. Microbiol. 74, 6280–6288. doi: 10.1128/AEM.00916-08.